Radiosensitivity and Chromosome Numbers in Strain L Mouse Cells in Tissue Culture

Radiosensitivity and chromosome numbers in strain L mouse cells in tissue culture.

The lethal effects of ionizing radiations on cells may be either genetic or nongenetic in origin and, if genetic, may be classified as dominant or recessive. If the radiosensitivity of an organism depends on its ploidy, it may be inferred that genetic damage is involved (1). Where such a dependence has been found, the relative importance of recessive and dominant lethal effects appears to accou...

Effect of Halogenated Pyrimidines on Radiosensitivity of Mouse Strain L Cells and Their Radioresistant Variant.

Incorporation of halogen analogs of pyrimidine into DNA of the mouse strain L cells enhanced their sensitivity to X-ray irradiation. The maximal radiosensitization was obtained either with 72 hours, incubation in the presence of BUdR alone or 24 hours' incubation in the presence of BUdR and aminopterin before X-ray irradiation. The replacement of thymidine by BUdR in these optimal conditions wa...

Survival of Bordetella pertussis in Tissue Culture of Animal Cells

An Unusual Behavior of Strain Hela Cells in Tissue Culture

The differentiation attained by emigrating embryo chick ceils cultivated under strips and sheets of dialysis cellphane (1-4) in multipurpose culture chambers (5) prompted us to make a similar study of malignant tissues and strains. One of the more provocative results obtained with HeLa cells (6, 7) is contained in this note. The environmental conditions resulting when sheets of cellophane are l...

The isolation and properties of a clonal tissue culture strain of pluripotent mouse teratoma cells.

A clonal tissue culture strain of pluripotent cells has been isolated from a transplantable teratoma of inbred strain of mice 129 Sv-Sl CP. This cell strain S1KR when re-inoculated into mice produces teratomas containing at least ten types of tissue. Sub-clones have been isolated and two types distinguished. (1) 'C-type' with a densely-piled in vitro growth. These are tumourigenetic and pluripo...